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19. | | RIGAMONTI, M. J. de S.; SANTOS, J. de A. dos; SOUZA, H. M. R. de. Alternância: uma educação para o desenvolvimento rural sustentável. In: BALSADI, O. V.; CRUZ, M. C. da; VERNE, M. C.; PEREIRA, V. da F.; SICOLI, A. H. (Ed.). Transferência de tecnologia e construção do conhecimento. Brasília, DF: Embrapa, 2013. Part. 5. Cap. 1. p. 305-314. O livro originou dos trabalhos apresentados no I Encontro Nacional sobre Metodologias para Transferência de conhecimentos e Tecnologia para o Desenvolvimento Rural Sustentável, Brasília, DF, 2011. Biblioteca(s): Embrapa Amapá. |
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20. | | SOUZA, H. M. F.; MACHADO, R. C. R.; MORENO, M. A.; ALVIM, P. de T. Avaliacao da resistencia a seca, em gramineas forrageiras, mediante determinacao do "ponto de compensacao hidrica". Revista Theobroma, Ilheus, v.12, n.3, p.141-147, jul./set. 1982. Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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Registro Completo
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
24/02/2022 |
Data da última atualização: |
01/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
MAKTURA, G. C.; PARANHOS, B. A. J.; SOUZA, H. M. |
Afiliação: |
GRAZIELLE C. MAKTURA; BEATRIZ AGUIAR GIORDANO PARANHOS, CPATSA; HENRIQUE MARQUES SOUZA. |
Título: |
RNAi in fruit flies (Diptera: Tephritidae): successes and challenges. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Journal of Applied Entomology, v. 145, n. 8, p. 740-756, 2021. |
DOI: |
10.1111/jen.12905 |
Idioma: |
Inglês |
Conteúdo: |
Fruit flies (Diptera: Tephritidae) are among the most economically important pests worldwide, causing direct damage to fruit crops through female oviposition and lar-val feeding. Currently, the most widely disseminated methods of control of fruit flies involve the adoption of cultural and biological techniques, like the sterile insect tech-nique, and the use of agrochemicals. However, the latter depend on the concomitant use of other techniques, especially chemical ones, which are the most successful, but also related to resistance, off-target effects and pollution, showing the need for new methods. RNA interference (RNAi) is a biotechnological tool that is capable of in-ducing post-transcriptional gene silencing using double-stranded RNA (dsRNA). The mechanism could be exploited in pest control associated with integrated pest man-agement, by targeting essential genes or by increasing the susceptibility of insects to insecticides. Here, we discuss the RNAi machinery and the uptake genes of the main Tephritidae species and their differences in comparison with other insects. Then, we investigate the international literature on the application of this tool to improve the sterile insect technique, enhance the insects? susceptibility to insecticides and modu-late their growth and development, demonstrating the sensibility of Tephritidae spe-cies to RNAi. Finally, we discuss the main challenges to the future use of RNAi as a method to control Tephritidae, such as delivery procedures, degradation and produc-tion of dsRNA, off-target effects, occurrence of refractoriness, and perspectives and shortages associated with its adoption that need to be considered. MenosFruit flies (Diptera: Tephritidae) are among the most economically important pests worldwide, causing direct damage to fruit crops through female oviposition and lar-val feeding. Currently, the most widely disseminated methods of control of fruit flies involve the adoption of cultural and biological techniques, like the sterile insect tech-nique, and the use of agrochemicals. However, the latter depend on the concomitant use of other techniques, especially chemical ones, which are the most successful, but also related to resistance, off-target effects and pollution, showing the need for new methods. RNA interference (RNAi) is a biotechnological tool that is capable of in-ducing post-transcriptional gene silencing using double-stranded RNA (dsRNA). The mechanism could be exploited in pest control associated with integrated pest man-agement, by targeting essential genes or by increasing the susceptibility of insects to insecticides. Here, we discuss the RNAi machinery and the uptake genes of the main Tephritidae species and their differences in comparison with other insects. Then, we investigate the international literature on the application of this tool to improve the sterile insect technique, enhance the insects? susceptibility to insecticides and modu-late their growth and development, demonstrating the sensibility of Tephritidae spe-cies to RNAi. Finally, we discuss the main challenges to the future use of RNAi as a method to control Tephritidae, such as delivery procedur... Mostrar Tudo |
Palavras-Chave: |
Inseto estéril; Interferência de RNA; Manejo integrado de pragas; RNAi; Silenciamento de genes; Técnica de inseto estéril; Tephritida. |
Thesagro: |
Controle Biológico; Mosca das Frutas. |
Thesaurus NAL: |
Biological control; Fruit flies. |
Categoria do assunto: |
O Insetos e Entomologia |
Marc: |
LEADER 02536naa a2200289 a 4500 001 2140376 005 2023-03-01 008 2021 bl uuuu u00u1 u #d 024 7 $a10.1111/jen.12905$2DOI 100 1 $aMAKTURA, G. C. 245 $aRNAi in fruit flies (Diptera$bTephritidae): successes and challenges.$h[electronic resource] 260 $c2021 520 $aFruit flies (Diptera: Tephritidae) are among the most economically important pests worldwide, causing direct damage to fruit crops through female oviposition and lar-val feeding. Currently, the most widely disseminated methods of control of fruit flies involve the adoption of cultural and biological techniques, like the sterile insect tech-nique, and the use of agrochemicals. However, the latter depend on the concomitant use of other techniques, especially chemical ones, which are the most successful, but also related to resistance, off-target effects and pollution, showing the need for new methods. RNA interference (RNAi) is a biotechnological tool that is capable of in-ducing post-transcriptional gene silencing using double-stranded RNA (dsRNA). The mechanism could be exploited in pest control associated with integrated pest man-agement, by targeting essential genes or by increasing the susceptibility of insects to insecticides. Here, we discuss the RNAi machinery and the uptake genes of the main Tephritidae species and their differences in comparison with other insects. Then, we investigate the international literature on the application of this tool to improve the sterile insect technique, enhance the insects? susceptibility to insecticides and modu-late their growth and development, demonstrating the sensibility of Tephritidae spe-cies to RNAi. Finally, we discuss the main challenges to the future use of RNAi as a method to control Tephritidae, such as delivery procedures, degradation and produc-tion of dsRNA, off-target effects, occurrence of refractoriness, and perspectives and shortages associated with its adoption that need to be considered. 650 $aBiological control 650 $aFruit flies 650 $aControle Biológico 650 $aMosca das Frutas 653 $aInseto estéril 653 $aInterferência de RNA 653 $aManejo integrado de pragas 653 $aRNAi 653 $aSilenciamento de genes 653 $aTécnica de inseto estéril 653 $aTephritida 700 1 $aPARANHOS, B. A. J. 700 1 $aSOUZA, H. M. 773 $tJournal of Applied Entomology$gv. 145, n. 8, p. 740-756, 2021.
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